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Blood was collected by submandibular puncture into 1 mL Eppendorf tubes, the plasma was separated and stored in 80 C until used

The trial used the efficacy estimand to measure the full glycaemic control potential of elecoglipron in patients who followed treatment as directed, providing the biological data needed to confirm optimal dosing for Phase III trials

Antibody pairs for mouse islets were mouse anti-mouse GLP-1R Ab Mab 7F38 (DSHB, 5 g/mL) and either rabbit anti-VAPB (A14703, Antibodies.com, 1:100), or rabbit anti-mouse SPHKAP (36690, kind gift from Dr Nicholas Vierra, University of Utah 32 , 1:100)

Gabriel, S

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